The German side managed to beat Arsenal on goal difference in Group F, while Zenit snuck into the knockout stage by knocking out Atletico Madrid in Group G.
Domestically, Dortmund’s season already looks in tatters as it sits 10 points behind rival Bayern Munich in the Bundesliga standings.
Zenit, on the other hand, is on course for fifth place in the Russian Premier League thanks to a narrow lead at the top of the table.
Tuesday’s clash will be the first time the two have met in a competitive match.
Ilkay Gundogan remains away from the visitors, while Neven Subotic is also unavailable due to injury.
Zenit have no new injuries ahead of the match.
Recent form
Zenith: WLLWLW
Dortmund: WWWWWL
Possible lineups
Zenith: Lodygin; Ansaldi, Hubocan, Lombaerts, Criscito; Faizulin, Witsel, Shirokov, Zyryanov, Hulk; Kerjakov
Dortmund: Weidenfeller; Piszczek, Friedrich, Papastathopoulos, Schmelzer; Aubameyang, Bender, Sahin, Grosskreutz; Mkhitaryan; Lewandowski
Topo Deportivo says: 0-2